Download PDF by Vic Pawlak: Conscientious Guide To Drug Abuse

By Vic Pawlak

4th revised variation staplebound alternate 1973 paperback vg+ . In inventory shipped from our united kingdom warehouse

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Details of the therapeutic chimeric antibodies summarized in Table 5 can be found in LARRICK and FRY (1991a). Recombinant Therapeutic Human Monoclonal Antibodies 31 Table 5. Therapeutic recombinant chimaeric antibodies Targets Reference Hepatitis surface antigen Human immunodeficiency virus antigen gp 120 CE7 T lymphocyte antigen CD4 Cancer antigens Carcinoembryonic antigen (CEA) LI et al. (1990) LIOU et al. (1989); CHIANG et aI. HEINRICH et al. 6, L6) Ovarian cancer Transferrin receptor Miscellaneous cancer cells BR96 BEIDLER et al.

J Immunol Methods 94:201-208 Kozbor D, Lagarde AE, Roder JC (1982) Human hybridomas constructed with antigen-specific Epstein virus - transformed cell lines. Proc Natl Acad Sci USA 79:6651-6655 Larrick JW, Fry KE (1991) Recombinant antibodies. Human Antib Hybrid 2: 172-189 Leader KA, Macht LM, Steers F, Kumpel BM, Elson CJ (1992) Antibody responses to the blood group antigen D in SCID mice reconstituted with human blood mononuclear cells. Immunology 76:222-234 Lewis AP, Parry N, Peakman TC, Scott Crowe J (1992) Rescue and expression of human immunoglobulin genes to generate functional human monoclonal antibodies.

The rearranged 59D8 heavy chain gene was cloned and combined in the expression vector pSV2gpt with sequence coding for a portion of the y 2b constant region and the catalytic P chain of t-PA. This construct was transfected into variant cells that lacked heavy chain and which were derived from the 59D8 hybridoma. The cells produced a 65 kDa heavy chain-t-PA fusion protein that was secreted in association with the 59D8 light chain in the form of a 170 kDa disulfide-linked dimer. Chromo- Recombinant Therapeutic Human Monoclonal Antibodies 33 genic substrate assays showed the fusion protein to have 70% of the peptidolytic activity of native t-PA and to activate plasminogen as efficiently as t-PA.

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