By Christophe Poncelet
Cet ouvrage de r?f?rence r?unit les principaux specialists nationaux et parfois internationaux des diff?rentes sp?cialit?s de los angeles replica. los angeles premi?re partie passe en revue les donn?es fondamentales de l. a. physiologie et retrace l'histoire de l'assistance m?dicale ? l. a. procr?ation. Sont abord?s ensuite les elements ?pid?miologiques et surtout m?dico-l?gaux. Un chapitre complet d?taille le diagnostique, le pronostique et les traitements chirurgicaux. Les options les plus r?centes d'assistance m?dicale ? l. a. procr?ation sont ensuite pass?es en revue, de m?me que les proc?d?s de cryopr?servation. L'ouvrage fait enfin une huge position aux consid?rations transversales (cancer, pathologies auto-immunes, implications ?thiques, etc.) mais essentielles autour de los angeles fertilit? et de l'infertilit?, et de son impression psychologique sur les undefined.
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References Zhang et al. (136) Hovatta et al. (139) Hovatta et al. (148) Louhio et al. (149) Rahimi et al. (140) Basal medium Waymouth _MEM/EBSS _MEM/EBSS/Waymouth EBSS EBSS DMEM Hreinsson et al. (150) _MEM Otala et al. (141) EBSS Serum, FSH Serum, FSH, I, IGF FBS, FSH Serum/HSA, ITS, FSH, cGMP, GDF-9 Serum, FSH, NAC Isachenko et al. (151) DMEM FCS, `-mercaptoethanol, FSH 21 Biron et al. (137) Serum-free medium FBS, FSH 28 Otala et al. (152) Ham’s F10 FSH, I, HSA, T, DHT, E 1 Scott et al. (146) Zhang et al.
The concentrations selected for use in the different media are usually chosen based on the ‘à la volonté’ principle. That is, the dose-response effects of a growth factor on follicles are measured after culture, and the results are used to select the concentration at which an optimal response is observed. All existing protocols for the culture of human follicles have used tissues from either adult (139, 152, 159) or fetal (136-138) ovaries. The development of follicle culture systems for larger mammals has been attempted in several species including goat (190), sheep (191), bovine (192, 193), and baboon (194).
Evidence that intra-oocyte cAMP hydrolysis is responsible for the occurrence of meiosis resumption in human oocytes can be found in the fact that inhibitors of the cAMP degrading phosphodiesterase (PDE) isoenzymes block spontaneous maturation in vitro (113-115). Nuclear and cytoplasmic oocyte maturation Nuclear maturation of the oocyte comprises nuclear modifications that take place during the resumption of meiosis to metaphase II. Upon LH surge, prophase I GV-stage oocytes undergo GVBD, or dissolution of the nuclear membrane, followed by condensation of chromatin in bivalents that align at the equator of the first meiotic spindle.