Polyamines: Methods and Protocols - download pdf or read online

By Anthony E. Pegg, Robert A. Casero Jr.

Recently, vital new findings within the polyamine box and a number of new experimental structures have revolutionized the examine of those ubiquitous mobile parts, crucial for regular progress and improvement. In Polyamines: equipment and Protocols, major researchers give a contribution an in depth number of updated laboratory concepts for the additional pursuit of polyamine research. the quantity delves into important matters equivalent to neoplasia reviews with animal versions and human sufferers, healing roles for polyamine inhibitors and analogs, polyamine metabolism and oxidative harm, polyamines as regulators of serious ion channels, in addition to polyamine delivery platforms and polyamine-responsive genes. Written within the hugely profitable Methods in Molecular Biology™ sequence layout, chapters comprise introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, simply reproducible laboratory protocols, and professional notes on troubleshooting and keeping off identified pitfalls.

Comprehensive and state-of-the-art, Polyamines: tools and Protocols presents a key source for all scientists pursuing the learn of this dynamic and critical element of mobile biology.

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Biochem J 366:663–671 120. Mitchell JL, Simkus CL, Thane TK, Tokarz P, Bonar MM, Frydman B, Valasinas AL, Reddy VK, Marton LJ (2004) Antizyme induction mediates feedback limitation of the incorporation of specific polyamine analogues in tissue culture. Biochem J 384:271–279 121. Soulet D, Gagnon B, Rivest S, Audette M, Poulin R (2004) A fluorescent probe of polyamine transport accumulates into intracellular acidic vesicles via a two-step mechanism. J Biol Chem 279:49355–49366 122. Belting M, Persson S, Fransson L-Å (1999) Proteoglycan involvement in polyamine uptake.

Since polyamines are ubiquitous components of plant and animal cells and are produced by intestinal microorganisms, there is substantial dietary exposure to polyamines (131). Uptake from this source is a significant potential problem leading to resistance to drugs that block endogenous polyamine synthesis which are used for cancer chemotherapy or chemoprevention (124, 132). Diets low in polyamines and supplemented with antibiotics to reduce synthesis by intestinal organisms have been designed to overcome this problem (133–135).

J Biol Chem 279:46008–46013 25. Igarashi K, Kashiwagi K (2006) Polyamine modulon in Escherichia coli: genes involved in the stimulation of cell growth by polyamines. J Biochem (Tokyo) 139:11–16 26. Uemura T, Higashi K, Takigawa M, Toida T, Kashiwagi K, Igarashi K (2009) Polyamine modulon in yeast-stimulation of COX4 synthesis by spermidine at the level of translation. Int J Biochem Cell Biol 41:2538–2545 27. Nishimura K, Okudaira H, Ochiai E, Higashi K, Kaneko M, Ishii I, Nishimura T, Dohmae N, 25 Kashiwagi K, Igarashi K (2009) Identification of proteins whose synthesis is preferentially enhanced by polyamines at the level of translation in mammalian cells.

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