By World Health Organization
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Citrated blood prepared in this way can be stored for about 8 days at 2-4°C. 4 TS and carefully dilute to volume. This diluted blood suspension (2% solution) can be used as long as the supernatant fluid remains clear and colourless. It must be stored at a cool temperature. 4 TS to make 100ml. This solution should be freshly prepared. 4 TS. Quality control methods for medicinal plant materials Table 3 Determination of haemolytic activity: serial dilution for the preliminary test Tube no. 4 TS and blood suspension (2%) using four test-tubes as shown in Table 3.
Use a piece of halide-free filterpaper about 4 cm square as the sample holder. Combustion of chlorine-containing residues Transfer an aliquot of the extract as prepared above onto the sample holder which is placed in a funnel using a solvent that will not dissolve the sample holder. Allow the solvent to evaporate. Wearing rubber gloves, remove the sample holder and its dry contents from the funnel, and fold it over and up to form a small packet, about 1 cm2 in area, and secure it in the centre of the platinum gauze.
If the solvent is anhydrous, water may remain absorbed in it leading to false results. It is therefore advisable to saturate the solvent with water before use. The test for loss on drying determines both water and volatile matter. Drying can be carried out either by heating to 100-105 °C or in a desiccator over phosphorus pentoxide R under atmospheric or reduced pressure at room temperature for a specified period of time. The desiccation method is especially useful for materials that melt to a sticky mass at elevated temperatures.